Difference between revisions of "Part:BBa K2961003"

Revision as of 10:26, 19 October 2021

gRNA / crRNA fixed-loop repeat for Cpf1 / Cas12a

Sequence (crRNA repeat) that all Lba Cas12a gRNAs need at the beginning. Also known as fixed loop

Characterization by iGEM21_GreatBay_SZ

- Group: iGEM21_GreatBay_SZ - Author: TianYi Huang

We attached the repeat sequence to guide RNA used to recognize their targets(https://parts.igem.org/Part:BBa_K3859013). The crRNA will bind with cas12a enzyme, then the target DNA will be recobgnised and unwound by cas12a. After target DNA binds with guide RNA, cas12a will be activated and cleveage the sequence around it. In our project, we primarily used Cas12a detection technology for the identification of spores. This decision was made with reference to the methodology used in the HOLMES system

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 10: / Line 10: @@
 - Author: TianYi Huang
-We attached the repeat sequence to guide RNA used to recognize their targets(BBa_K3859013). The crRNA will bind with cas12a enzyme, then the target DNA will be recobgnised and unwound by cas12a. After target DNA binds with guide RNA, cas12a will be activated and cleveage the sequence around it.
+We attached the repeat sequence to guide RNA used to recognize their targets(https://parts.igem.org/Part:BBa_K3859013). The crRNA will bind with cas12a enzyme, then the target DNA will be recobgnised and unwound by cas12a. After target DNA binds with guide RNA, cas12a will be activated and cleveage the sequence around it.
 In our project, we primarily used Cas12a detection technology for the identification of spores. This decision was made with reference to the methodology used in the HOLMES system