Difference between revisions of "Part:BBa K3963001"
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The plasmid is combined by the Backbone from the pBWB162 plasmid (BBa_K3963000) and cloned by restriction based cloning with the insert beta-Agarase YM01-3 (BBa_K2094002). mCherry protein was cut out. The plasmid still contains the lacI behind lacIq promoter and a kanamycin selection marker. | The plasmid is combined by the Backbone from the pBWB162 plasmid (BBa_K3963000) and cloned by restriction based cloning with the insert beta-Agarase YM01-3 (BBa_K2094002). mCherry protein was cut out. The plasmid still contains the lacI behind lacIq promoter and a kanamycin selection marker. | ||
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+ | ===Design=== | ||
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+ | [[File:T--Heidelberg--beta-agarase--NT--Map.png|thumbnail|center|500px|<b>Figure 1 Plasmid design</b>]] | ||
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+ | ===Experiments and results=== | ||
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Revision as of 10:11, 19 October 2021
pBAV1k-lacI-Trc-beta-agarase YM01-3
The plasmid is combined by the Backbone from the pBWB162 plasmid (BBa_K3963000) and cloned by restriction based cloning with the insert beta-Agarase YM01-3 (BBa_K2094002). mCherry protein was cut out. The plasmid still contains the lacI behind lacIq promoter and a kanamycin selection marker.
Design
Experiments and results
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 3183
Illegal AgeI site found at 3220 - 1000COMPATIBLE WITH RFC[1000]