Difference between revisions of "Part:BBa K3994008"

 
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<partinfo>BBa_K3994008 short</partinfo>
 
<partinfo>BBa_K3994008 short</partinfo>
  
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PyeaR_HrpR
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promoter
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=== Profile ===
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==== Name: PyeaR_HrpR ====
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==== Base Pairs: 1223 bp ====
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==== Origin: Synthetic ====
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==== Properties: A coding sequence of HrpR protein. ====
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=== Usage and Biology ===
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==== Background ====
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Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory disease of unknown etiology, including ulcerative colitis (CD) and Crohn’s disease (CD). This chronic disease, which is prone to repeated deterioration, currently lacks unified diagnostic and treatment standards, and is posing a great threat to public health. Drug therapy (anti-inflammatory drugs) is the preferred treatment for IBD. However, studies in the past 10 years have found that 30-50% of IBD patients do not respond to anti-TNF treatment. In addition, after long-term use of anti-inflammatory drugs, the patient's intestinal microbial status changes over time, and the effect may be lost due to drug resistance. Therefore, we need to seek help from other treatments for IBD. HrpR protein binds to HrpS protein forming a complex and then triggering the transcription of promoter hrpL. This part can sense NO3- to release the substance R.
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=== Construct design ===
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HrpR is a key functional factor under PyeaR promoter (Figure 1). And this part is inserted into plasmid. Its sequence is shown in Figure 2
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[[File:T--NOFLS YZ--BBa K3994008-Figure1.png|500px|thumb|center|Figure1 PyeaR_HrpR box...]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:38, 19 October 2021


PyeaR_HrpR


promoter

Profile

Name: PyeaR_HrpR

Base Pairs: 1223 bp

Origin: Synthetic

Properties: A coding sequence of HrpR protein.

Usage and Biology

Background

Inflammatory bowel disease (IBD) is a chronic intestinal inflammatory disease of unknown etiology, including ulcerative colitis (CD) and Crohn’s disease (CD). This chronic disease, which is prone to repeated deterioration, currently lacks unified diagnostic and treatment standards, and is posing a great threat to public health. Drug therapy (anti-inflammatory drugs) is the preferred treatment for IBD. However, studies in the past 10 years have found that 30-50% of IBD patients do not respond to anti-TNF treatment. In addition, after long-term use of anti-inflammatory drugs, the patient's intestinal microbial status changes over time, and the effect may be lost due to drug resistance. Therefore, we need to seek help from other treatments for IBD. HrpR protein binds to HrpS protein forming a complex and then triggering the transcription of promoter hrpL. This part can sense NO3- to release the substance R.

Construct design

HrpR is a key functional factor under PyeaR promoter (Figure 1). And this part is inserted into plasmid. Its sequence is shown in Figure 2


Figure1 PyeaR_HrpR box...


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 669
    Illegal AgeI site found at 142
    Illegal AgeI site found at 256
    Illegal AgeI site found at 262
    Illegal AgeI site found at 901
  • 1000
    COMPATIBLE WITH RFC[1000]