Difference between revisions of "Part:BBa K3991000"

Revision as of 07:37, 19 October 2021

ArsD

Profile

Name: ArsD

Base Pairs: 1200 bp

Origin: Escherichia coli (strain: LST424C, nat-host: Homo sapiens)

Properties: Gene technology for protecting patented bacterial strains

ArsD is a trans-acting repressor of the arsRDABC operon that confers resistance to arsenicals and antimonials in Escherichia coli.

Experimental approach

Production, purification, and sequcing analysis of recombinant ArsD-amilGFP

Figure 1: Gel Electrophoresis Results of PCR of amilGFP Genes..

Figure 1 shows gel electrophoresis results of amilGFP PCR. Column M is a 2K marker ladder. Columns 1-6 are PCR products of amilGFP genes All 1-6 columns displayed successful results at 700bp which could be used for later experiments.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3991000 short</partinfo>
-ArsD
+== Profile ==
+==== Name: ArsD ====
+==== Base Pairs: 1200 bp ====
+==== Origin: Escherichia coli (strain: LST424C, nat-host: Homo sapiens) ====
+==== Properties: Gene technology for protecting patented bacterial strains ====
+ArsD is a trans-acting repressor of the arsRDABC operon that confers resistance to arsenicals and antimonials in Escherichia coli.
+== Experimental approach ==
+=== Production, purification, and sequcing analysis of recombinant ArsD-amilGFP ===
+[[File:T--Shanghai United--result01.jpg|500px|thumb|center|Figure 1: Gel Electrophoresis Results of PCR of amilGFP Genes..]]
+Figure 1 shows gel electrophoresis results of amilGFP PCR. Column M is a 2K marker ladder. Columns 1-6 are PCR products of amilGFP genes
+All 1-6 columns displayed successful results at 700bp which could be used for later experiments.
 <!-- Add more about the biology of this part here