Difference between revisions of "Part:BBa K4099004"

 
 
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<partinfo>BBa_K4099004 short</partinfo>
 
<partinfo>BBa_K4099004 short</partinfo>
  
LSEI-2094
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=== Profile ===
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==== Name: LSEI-2094 ====
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==== Base Pairs: 1599bp ====
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==== Origin: Lactobacillus casei, genome ====
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==== Properties: A coding sequence of restriction endonuclease LSEI-2094 ====
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=== Usage and Biology ===
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This part is a coding sequence of LSEI-2094 from Lactobacillus casei. Restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes. Restriction enzymes are commonly classified into five types. These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses. Inside a prokaryote, the restriction enzymes selectively cut up foreign DNA in a process called restriction digestion; meanwhile, host DNA is protected by a modification enzyme (a methyltransferase) that modifies the prokaryotic DNA and blocks cleavage. Together, these two processes form the restriction modification system.
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Latest revision as of 06:36, 19 October 2021


LSEI-2094

Profile

Name: LSEI-2094

Base Pairs: 1599bp

Origin: Lactobacillus casei, genome

Properties: A coding sequence of restriction endonuclease LSEI-2094

Usage and Biology

This part is a coding sequence of LSEI-2094 from Lactobacillus casei. Restriction endonuclease is an enzyme that cleaves DNA into fragments at or near specific recognition sites within molecules known as restriction sites. Restriction enzymes are one class of the broader endonuclease group of enzymes. Restriction enzymes are commonly classified into five types. These enzymes are found in bacteria and archaea and provide a defense mechanism against invading viruses. Inside a prokaryote, the restriction enzymes selectively cut up foreign DNA in a process called restriction digestion; meanwhile, host DNA is protected by a modification enzyme (a methyltransferase) that modifies the prokaryotic DNA and blocks cleavage. Together, these two processes form the restriction modification system.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1509
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 177
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]