Difference between revisions of "Antibiotic Resistance"

 
 
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Antibiotic Resistance of the pSP1Ax3 Plasmids
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Antibiotic Resistance of the pSB1Ax3 Plasmids
  
 
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Latest revision as of 22:54, 5 May 2009

Antibiotic Resistance of the pSB1Ax3 Plasmids

Tet Kan Cm
pSB1AT3 150ug/ml 5 ug/ml 3.5 ug/ml
pSB1AK3 1.5 ug/ml 5000 ug/ml 3.5 ug/ml
pSB1AC3 1.5 ug/ml 5 ug/ml 3500 ug/ml
Normal Plates (1 L volume)
Antibiotic 3ml 1 ml 1 ml
Antibiotic @5 mg/ml @ 50 mg/ml 35 mg/ul
Normal 15 ug/ml 50 ug/ml 35 ug/ml

The normal plate recipe is the specified volume of antibiotic at the specified antibiotic strength in one Liter of agar broth. This results in the specified concentration of antibiotic in the plates.

In all cases, the antibiotic concentration is selected to be 10 times the amount required to select against cells that have been transformed with the wrong plasmid.

In all cases, cells transformed with the correct plasmid are able to tolerate at least 10 times this concentration of antibiotic.

Antibiotic resistances were measured by Tom Knight and Steve Payne (6/6/2005).