Difference between revisions of "Part:BBa J70303"

 
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<partinfo>BBa_J70303 short</partinfo>
 
<partinfo>BBa_J70303 short</partinfo>
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Assembly of pSCI2 plasmid origin and cat cassette into E. coli plasmid pSB1T3.
 
Assembly of pSCI2 plasmid origin and cat cassette into E. coli plasmid pSB1T3.
  
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===Usage and Biology===
 
===Usage and Biology===
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MIC in E. coli DH10B cells is 1 ug/ml of chloramphenicol.
  
 
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Revision as of 22:52, 5 May 2009

Test Cm resistance plasmid for Mesoplasma florum

Assembly of pSCI2 plasmid origin and cat cassette into E. coli plasmid pSB1T3.

Usage and Biology

MIC in E. coli DH10B cells is 1 ug/ml of chloramphenicol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 372