Difference between revisions of "Part:BBa K3822004"

 
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3822004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3822004 SequenceAndFeatures</partinfo>
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=Result=
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According to the advice given by Dr. Xiaoqing, "Multiple genetic testing can improve the accuracy and sensitivity of the product, but too many genes will bring about cost increase, complexity, and other negative effects". We decided to add another gene SDC2，together with TFPI2， as a detector to determine whether it is colorectal cancer.
  
 +
Same to TFPI2, we conducted a lot of literature research, selected a methylation-enriched region of SDC2 and designed methylation-specific primers. T7 promoter was added to its forward primer and trigger primer was added to its reverse primer. The primer we designed are below:
  
 +
SDC2-M: methylation specific primer
 +
 +
F: 5′- <font color="red">TAATACGACTCACTATAGGG</font>GTGTAGAAATTAATAAGTGAGAGGG-3′
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R: 5′- <font color="blue">TATGTAATTGATTTGGCTTCTGTTAGTTTCATATTT</font>CTACTTACAACCAAAACAAAAC-3′
 +
 +
PCR product：264bp
 +
(The red is T7 and the blue is Trigger)
 +
 +
Due to time reasons, we did not conduct experimental verification, but the exact same experiment as TFPI2 determines the success of the experiment.
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3822004 parameters</partinfo>
 
<partinfo>BBa_K3822004 parameters</partinfo>
 
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Latest revision as of 04:09, 19 October 2021


Hypermethylated region of SDC2

The sequence of Hypermethylated region of SDC2

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Result

According to the advice given by Dr. Xiaoqing, "Multiple genetic testing can improve the accuracy and sensitivity of the product, but too many genes will bring about cost increase, complexity, and other negative effects". We decided to add another gene SDC2，together with TFPI2， as a detector to determine whether it is colorectal cancer.

Same to TFPI2, we conducted a lot of literature research, selected a methylation-enriched region of SDC2 and designed methylation-specific primers. T7 promoter was added to its forward primer and trigger primer was added to its reverse primer. The primer we designed are below:

SDC2-M: methylation specific primer

F: 5′- TAATACGACTCACTATAGGGGTGTAGAAATTAATAAGTGAGAGGG-3′

R: 5′- TATGTAATTGATTTGGCTTCTGTTAGTTTCATATTTCTACTTACAACCAAAACAAAAC-3′

PCR product：264bp (The red is T7 and the blue is Trigger)

Due to time reasons, we did not conduct experimental verification, but the exact same experiment as TFPI2 determines the success of the experiment.