Difference between revisions of "Part:BBa K2717000"
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<partinfo>BBa_K2717000 parameters</partinfo> | <partinfo>BBa_K2717000 parameters</partinfo> | ||
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| + | <font size="4"><b>BNUZ-China 2021</b></font> | ||
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| + | To explore whether GDH can effectively promote the proliferation of engineered bacteria without IPTG induction, we have drawn the growth curve of engineered bacteria and normal bacteria. From the growth curve (Figure 1) we can learn that compared with E. coli that had not overexpressed GDH, our engineered bacteria had a certain proliferation advantage, although the effect is not obvious. We subsequently established a mathematical model of intestinal colony competition. The results showed that although GDH slightly enhanced the colonization advantage of engineered bacteria, it could be eventually well colonized in the intestinal tract. | ||
| + | [[File:BNUZ-gdh1.png|420px|thumb|left|Figure 1. GDH overexpression promotes cell growth]] | ||
| + | [[File:BNUZ-gdh2.jpeg|440px|thumb|right|Figure 2. GDH overexpression promotes cell growth (adapted, with permission from BNU-China 2019)]] | ||
Revision as of 03:34, 19 October 2021
gdh
Sequence of glucose dehydrogenase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1542
- 1000COMPATIBLE WITH RFC[1000]
BNUZ-China 2021
To explore whether GDH can effectively promote the proliferation of engineered bacteria without IPTG induction, we have drawn the growth curve of engineered bacteria and normal bacteria. From the growth curve (Figure 1) we can learn that compared with E. coli that had not overexpressed GDH, our engineered bacteria had a certain proliferation advantage, although the effect is not obvious. We subsequently established a mathematical model of intestinal colony competition. The results showed that although GDH slightly enhanced the colonization advantage of engineered bacteria, it could be eventually well colonized in the intestinal tract.
