Difference between revisions of "Part:BBa K3738009"

Revision as of 02:47, 19 October 2021

MlrA (Microcystinase) Composite Part

MlrA, or microcystinase, cleaves the peptide backbone of MC-LR cyanotoxins produced by microcystis aeruginosa cyanobacteria. This crucial step results in the peptide losing the cyclic structure, and therefore the majority of its toxicity. Further degradation continues in an enzymatic pathway by interactions with other peptidolytic enzymes from the microcystinase gene cluster. This composite part includes the IPTG-inducible T7 promoter (BBa_J64997), RBS BBa_B0034, microcystinase coding region BBa_K3738019, and double terminator BBa_B0015.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 556
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3738009 short</partinfo>
-MlrA, or microcystinase, cleaves the peptide backbone of MC-LR cyanotoxins produced by microcystis aeruginosa cyanobacteria. This crucial step results in the peptide losing the cyclic structure, and therefore the majority of its toxicity. Further degradation continues in an enzymatic pathway by interactions with other peptidolytic enzymes from the microcystinase gene cluster. This composite part includes the IPTG-inducible T7 promoter (BBa_J64997), RBS BBa_B0034, microcystinase coding region BBa_K1907001, and double terminator BBa_B0015.
+MlrA, or microcystinase, cleaves the peptide backbone of MC-LR cyanotoxins produced by microcystis aeruginosa cyanobacteria. This crucial step results in the peptide losing the cyclic structure, and therefore the majority of its toxicity. Further degradation continues in an enzymatic pathway by interactions with other peptidolytic enzymes from the microcystinase gene cluster. This composite part includes the IPTG-inducible T7 promoter (BBa_J64997), RBS BBa_B0034, microcystinase coding region BBa_K3738019, and double terminator BBa_B0015.
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