Difference between revisions of "Part:BBa K3493000"
 
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CDS of a family 66 dextranase from the psychrophile organism Gelidibacter algens, codon optimized for Escherichia coli.
 
CDS of a family 66 dextranase from the psychrophile organism Gelidibacter algens, codon optimized for Escherichia coli.
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EDIT 2021-10-18 : We expressed this dextranase at 18 °C for 18 hours with 1 mM IPTG, and we were able to purify it using IMAC chromatography. Using micro Nelson-Somogyi assays, we determined that optimal pH is at 6.5 and the optimal temperature at 40 °C. Since the enzyme is more stable at temperature under 20 °C, we did our assays at 18 °C which was sufficient later on to prove it reduced the viscosity of ropy maple syrup by degrading dextran.
  
 
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Latest revision as of 01:09, 19 October 2021


G. algens dextranase CDS

CDS of a family 66 dextranase from the psychrophile organism Gelidibacter algens, codon optimized for Escherichia coli.

EDIT 2021-10-18 : We expressed this dextranase at 18 °C for 18 hours with 1 mM IPTG, and we were able to purify it using IMAC chromatography. Using micro Nelson-Somogyi assays, we determined that optimal pH is at 6.5 and the optimal temperature at 40 °C. Since the enzyme is more stable at temperature under 20 °C, we did our assays at 18 °C which was sufficient later on to prove it reduced the viscosity of ropy maple syrup by degrading dextran.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 385
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 385
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 385
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]