Difference between revisions of "Part:BBa K3790225:Design"
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The following primers were used for constructing this part. | The following primers were used for constructing this part. | ||
<pre> | <pre> | ||
| − | + | F_A1csiD1_1035 TTTTCAGAAATCTAGATATTTTTAGATTAACTAGAGAAAGAGGAGAAATACTAGATGC | |
| − | + | R_A1csiD1_1907 AATATCTAGATTTCTGAAAAATGCGCACACTCCCACTCCACCTGAACC | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
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</pre> | </pre> | ||
Latest revision as of 00:14, 19 October 2021
GFP controlled by σS promoter csiD
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 20
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 20
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 20
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 707
Design Notes
The following primers were used for constructing this part.
F_A1csiD1_1035 TTTTCAGAAATCTAGATATTTTTAGATTAACTAGAGAAAGAGGAGAAATACTAGATGC R_A1csiD1_1907 AATATCTAGATTTCTGAAAAATGCGCACACTCCCACTCCACCTGAACC