Difference between revisions of "Part:BBa K3889132"

 
 
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Cassette for checking BBa_B0010 and Bacillus subtilis native terminator nagP using dual reporter system.
 
Cassette for checking BBa_B0010 and Bacillus subtilis native terminator nagP using dual reporter system.
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[[File:T--IISER-Tirupati India--Improvement3.jpeg|690px]]<br>
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Fig 1. Spacer replaced from <partinfo>BBa_K3889130</partinfo> by <partinfo>BBa_B0010</partinfo>
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Formulae for terminator efficiency [1]:
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[[File:T--IISER-Tirupati_India--Eq1.png]]
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where
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mCherry<sub>0</sub> &#8594; mCherry produced by device without terminator
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sfGFP<sub>0</sub> &#8594; sfGFP produced by device without terminator
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Using the device (<partinfo>BBa_K3889130</partinfo>) without any changes, TE<sub>Device</sub> can be calculated, which gives the expression of mCherry in the absence of a terminator.
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[[File:T--IISER-Tirupati_India--Eq2.png]]
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where
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mCherry &#8594; mCherry produced by the device with terminator
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sfGFP &#8594; sfGFP produced by the device with terminator
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and TE &#8594; is the terminator efficiency of the <partinfo>BBa_K3889070</partinfo>
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<partinfo>BBa_K3889132 parameters</partinfo>
 
<partinfo>BBa_K3889132 parameters</partinfo>
 
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===References===
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1. Gale, G. A. R., Wang, B., & McCormick, A. J. (2021). Evaluation and Comparison of the Efficiency of Transcription Terminators in Different Cyanobacterial Species. Frontiers in Microbiology, 11. https://doi.org/10.3389/fmicb.2020.624011

Latest revision as of 19:58, 18 October 2021


Bacillus subtilis BBa_B0010-nagP fused terminator check cassette

Cassette for checking BBa_B0010 and Bacillus subtilis native terminator nagP using dual reporter system. T--IISER-Tirupati India--Improvement3.jpeg
Fig 1. Spacer replaced from BBa_K3889130 by BBa_B0010

Formulae for terminator efficiency [1]:

T--IISER-Tirupati India--Eq1.png

where

mCherry0 → mCherry produced by device without terminator

sfGFP0 → sfGFP produced by device without terminator

Using the device (BBa_K3889130) without any changes, TEDevice can be calculated, which gives the expression of mCherry in the absence of a terminator.

T--IISER-Tirupati India--Eq2.png

where

mCherry → mCherry produced by the device with terminator

sfGFP → sfGFP produced by the device with terminator

and TE → is the terminator efficiency of the BBa_K3889070


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 121


References

1. Gale, G. A. R., Wang, B., & McCormick, A. J. (2021). Evaluation and Comparison of the Efficiency of Transcription Terminators in Different Cyanobacterial Species. Frontiers in Microbiology, 11. https://doi.org/10.3389/fmicb.2020.624011