Difference between revisions of "Part:BBa K3799004:Design"

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https://pubmed.ncbi.nlm.nih.gov/31054335/
 
https://pubmed.ncbi.nlm.nih.gov/31054335/
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Pérez-Ibarreche M, Field D, Ross RP, Hill C. A Bioengineered Nisin Derivative To Control Streptococcus uberis Biofilms. Appl Environ Microbiol. 2021 Jul 27;87(16):e0039121. doi: 10.1128/AEM.00391-21. Epub 2021 Jul 27. PMID: 34105992; PMCID: PMC8315181.
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Field D, Blake T, Mathur H, O' Connor PM, Cotter PD, Paul Ross R, Hill C. Bioengineering nisin to overcome the nisin resistance protein. Mol Microbiol. 2019 Mar;111(3):717-731. doi: 10.1111/mmi.14183. Epub 2019 Feb 12. PMID: 30537404.

Latest revision as of 19:47, 18 October 2021


Nisin PV, A synthetic antimicrobial peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The DNA sequence is synthetically produced and codon optimized for Ecoli host

Source

Nisin PV is completely synthetic in origin and is a mutated variant of Nisin A naturally produced by Lactococcus lactis,

References

https://onlinelibrary.wiley.com/doi/10.1111/mmi.14183

https://annalsmicrobiology.biomedcentral.com/articles/10.1007/s13213-013-0679-9

https://pubmed.ncbi.nlm.nih.gov/31054335/

Pérez-Ibarreche M, Field D, Ross RP, Hill C. A Bioengineered Nisin Derivative To Control Streptococcus uberis Biofilms. Appl Environ Microbiol. 2021 Jul 27;87(16):e0039121. doi: 10.1128/AEM.00391-21. Epub 2021 Jul 27. PMID: 34105992; PMCID: PMC8315181.

Field D, Blake T, Mathur H, O' Connor PM, Cotter PD, Paul Ross R, Hill C. Bioengineering nisin to overcome the nisin resistance protein. Mol Microbiol. 2019 Mar;111(3):717-731. doi: 10.1111/mmi.14183. Epub 2019 Feb 12. PMID: 30537404.