Difference between revisions of "Part:BBa K3799004"
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<partinfo>BBa_K3799004 short</partinfo> | <partinfo>BBa_K3799004 short</partinfo> | ||
− | This antimicrobial peptide is a bioengineered variant of Nisin A, produced naturally in Lactococcus lactis. Mutation of Serine to proline at position 29 (S29P) combined with isoleucine to valine | + | Description - This antimicrobial peptide is a bioengineered variant of Nisin A, produced naturally in Lactococcus lactis. Nisin A is a 34 amino acid peptide with Serine in the 29th position and Isoleucine in the 30th position. Mutation of Serine to proline at position 29 (S29P) combined with isoleucine to valine at position 30 (I30V) helps in making Nisin PV. Due to this mutation in the 29th and 30th positions Nisin PV is resistant to Nisin Resistance Protein (NSR) which is expressed on the cell surface of certain pathogenic strains like Streptococcus agalactiae, Streptococcus uberis. NSR is responsible for inactivating nisin by proteolytically cleaving the peptide bond between MeLan28 and Ser29 resulting in a truncated nisin (nisin1–28). Nisin PV also shows enhanced antimicrobial activity against Streptococcus uberis by inhibiting biofilm formation and decreasing its viability. |
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Revision as of 19:46, 18 October 2021
Nisin PV, A synthetic antimicrobial peptide
Description - This antimicrobial peptide is a bioengineered variant of Nisin A, produced naturally in Lactococcus lactis. Nisin A is a 34 amino acid peptide with Serine in the 29th position and Isoleucine in the 30th position. Mutation of Serine to proline at position 29 (S29P) combined with isoleucine to valine at position 30 (I30V) helps in making Nisin PV. Due to this mutation in the 29th and 30th positions Nisin PV is resistant to Nisin Resistance Protein (NSR) which is expressed on the cell surface of certain pathogenic strains like Streptococcus agalactiae, Streptococcus uberis. NSR is responsible for inactivating nisin by proteolytically cleaving the peptide bond between MeLan28 and Ser29 resulting in a truncated nisin (nisin1–28). Nisin PV also shows enhanced antimicrobial activity against Streptococcus uberis by inhibiting biofilm formation and decreasing its viability.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]