Difference between revisions of "Part:BBa K3900021"
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'''Measurement''' | '''Measurement''' | ||
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| − | The improved pH stability was determined by measuring the relative fluorescence of mRuby3 and mCRISPRed at different pH conditions. The fluorescence proteins were expressed and purified via IMAC beforehand. | + | The improved pH stability was determined by measuring the relative fluorescence of mRuby3 and mCRISPRed at different pH conditions. The fluorescence proteins were expressed in <I>Escherichia coli</I> BL21 DE3 and purified via IMAC beforehand. |
Revision as of 19:38, 18 October 2021
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mCRISPRed - improvement of mRuby3 |
This part is an improved version of the red fluorescent protein mRuby3 (BBa_M50009), that displays an enhanced stability at low pH conditions. The original part can be found at: https://parts.igem.org/Part:BBa_M50009
Measurement
The improved pH stability was determined by measuring the relative fluorescence of mRuby3 and mCRISPRed at different pH conditions. The fluorescence proteins were expressed in Escherichia coli BL21 DE3 and purified via IMAC beforehand.