Difference between revisions of "Part:BBa K3889130"

 
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<partinfo>BBa_K3889130 short</partinfo>
 
<partinfo>BBa_K3889130 short</partinfo>
  
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Cassette for use as a reference level due to the presence of a spacer sequence for checking terminator efficiency in Bacillus subtilis.
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This circuit consists of a dual reporter system in order to check terminator efficiency in <i>Bacillus subtilis</i>.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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*<h4>Measuring efficiency:</h4>
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The experiment is divided into two cassettes: one reference and a test cassette containing a terminator whose efficiency needs to be determined, as shown by Gale et al.[1].
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[[File:T--IISER-Tirupati India--Improvement1.jpeg|690px]]<br>
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Fig 1. Spacer Cassette for Terminator check
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[[File:T--IISER-Tirupati India--contributiontermtobechecked 01.jpg|690px]]<br>
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Fig 2. Spacer replaced by terminator to be checked
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The reference(Fig 1) and the test cassette (Fig 2) provide the expression levels of both the fluorescent proteins which could be compared to tell us how efficiently the terminator is working.
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Formulae for terminator efficiency [1]:
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[[File:T--IISER-Tirupati_India--Eq1.png]]
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where
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mCherry<sub>0</sub> &#8594; mCherry produced by device without terminator
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sfGFP<sub>0</sub> &#8594; sfGFP produced by device without terminator
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Using the device without any changes, TE<sub>Device</sub> can be calculated which gives the expression of mCherry in the absence of a terminator.
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[[File:T--IISER-Tirupati_India--Eq2.png]]
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where
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mCherry &#8594; mCherry produced by the device with terminator
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sfGFP &#8594; sfGFP produced by the device with terminator
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<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3889130 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3889130 SequenceAndFeatures</partinfo>

Revision as of 17:38, 18 October 2021


Spacer Cassette for Terminator check in Bacillus subtilis

This circuit consists of a dual reporter system in order to check terminator efficiency in Bacillus subtilis.

Usage and Biology

  • Measuring efficiency:

The experiment is divided into two cassettes: one reference and a test cassette containing a terminator whose efficiency needs to be determined, as shown by Gale et al.[1].

T--IISER-Tirupati India--Improvement1.jpeg
Fig 1. Spacer Cassette for Terminator check

T--IISER-Tirupati India--contributiontermtobechecked 01.jpg
Fig 2. Spacer replaced by terminator to be checked


The reference(Fig 1) and the test cassette (Fig 2) provide the expression levels of both the fluorescent proteins which could be compared to tell us how efficiently the terminator is working.

Formulae for terminator efficiency [1]:

T--IISER-Tirupati India--Eq1.png

where

mCherry0 → mCherry produced by device without terminator

sfGFP0 → sfGFP produced by device without terminator

Using the device without any changes, TEDevice can be calculated which gives the expression of mCherry in the absence of a terminator.

T--IISER-Tirupati India--Eq2.png

where

mCherry → mCherry produced by the device with terminator

sfGFP → sfGFP produced by the device with terminator


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 121