Difference between revisions of "Part:BBa K3889092"

Revision as of 17:25, 18 October 2021

XylR repressor(w/o stop codons)

XylR is a xylose based repressor from Bacillus subtilis . It is a part of XylR regulon where it functions to regulate xylose utilization. It can repress Pxyl (BBa_K143014) in absence of xylose. This is a modified version of XylR (BBa_K143036) from which stop codon is omitted from which either fusion proteins can be made or can be fused with a linker like P2A linker (BBa_K3889069) to coexpress fluorescent proteins to report protein production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3889092 short</partinfo>
-Modified xylR repressor for fusion with cleavage peptide or tag.
+XylR is a xylose based repressor from <i>Bacillus subtilis</i> . It is a part of XylR regulon where it functions to regulate xylose utilization. It can repress Pxyl (<partinfo>BBa_K143014</partinfo>) in absence of xylose.
+This is a modified version of XylR (<partinfo>BBa_K143036</partinfo>) from which stop codon is omitted from which either fusion proteins can be made or can be fused with a linker like P2A linker (<partinfo>BBa_K3889069</partinfo>) to coexpress fluorescent proteins to report protein production.
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