Difference between revisions of "Part:BBa K3952001:Design"
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===Design Notes===
 
===Design Notes===
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This is the negative control of the APOB gene that will be used within a Flappase genetic testing system.
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This is the positive control of the APOB gene that will be used within a Flappase genetic testing system.
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A SpaI restriction site was found at 853.  To adhere to RCF1000 assembly, a silent mutation was applied to the codon located within 855 and 857. The original codon was AGA and was altered to AGG.
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A SpeI restriction site was found at position 853. To adhere to RCF1000 assembly, a silent mutation was applied to the codon located within positions 855 and 857. The original codon was AGA and was altered to AGG.
  
 
===Source===
 
===Source===

Revision as of 17:10, 18 October 2021


APOB-100 (Cholesterol Deficiency Allele)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 330
    Illegal XhoI site found at 591
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 853


Design Notes

This is the positive control of the APOB gene that will be used within a Flappase genetic testing system.


A SpeI restriction site was found at position 853. To adhere to RCF1000 assembly, a silent mutation was applied to the codon located within positions 855 and 857. The original codon was AGA and was altered to AGG.

Source

The source of the APOB-100 sequence is the genomic sequence from Bos taurus, apolipoprotein B genome

References

Through the assistance provide by Team Rochester, we were able to acquire the ERV2-1 sequence from their subscription to Repbase. Repbase is a database consisting of repetitive DNA elements.