Difference between revisions of "Part:BBa K3952001"

Revision as of 17:08, 18 October 2021

APOB-100 (Cholesterol Deficiency Allele)

Located within chromosome 11 of the Bos taurus genome, the APOB gene codes for apolipoprotein B, a protein involved in the transport of cholesterol in the bloodstream. Studies have reported an inheritable allele within the APOB gene that causes cholesterol deficiency (CD) in cattle. This part contains the cholesterol deficiency allele in the APOB gene with a transposable insertion between nucleotides CT at positions 221, 222. Part: BBa_K3952000 is the wild-type version of the APOB gene that contains nucleotides CT at positions 221, 222.

The APOB gene (Gene ID 494004) was located within the genomic DNA sequence of the Bos taurus, using the NCBI reference assembly.

Usage and Biology

The purpose of this part is to serve as a positive control for a Flappase genetic testing system. The system requires the amplification of DNA around the polymorphism, therefore the sequence provide is only a fragment of the Bos taurus genome. The sequence was cut in order to only showcase where the allele would be present (exon 5) but also allow ease of cloning.

This part does contain the APOB inheritable allele. The allele is created by an insertion of a transposable element (ERV2-1) into the APOB gene. The insertion occurs between the 24th and 25th nucleotide of exon 5, which is between nucleotides CT at positions 221, 222. The insertion polymorphism results in cholesterol deficiency (CD) within cattle.

Sequence and Features