Difference between revisions of "Part:BBa K3735002"

Latest revision as of 16:48, 18 October 2021

Coding sequence

Native SsuE was a homodimeric enzyme of 58.4 kDa, which catalyzed an NAD(P)H-dependent reduction of FMN, but it was also able to reduce FAD or riboflavin. We adjusted its expression intensity in engineered bacteria, in order to enhance the bioreduction ability and enhance its tolerance to selenite, finally improve the yield of SeNPs.

Gene ssuE comes from the ssuEADCB gene cluster, which is required for the utilization of alkanesulfonates as sulfur sources, and is expressed under conditions of sulfate or cysteine starvation. Escherichia coli BL21 also has this gene cluster. The gene sequence of this part was obtained from NCBI and its GenBank Accession number is: 8182327.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 135
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 15:59, 16 October 2021 (view source) YihengYang (Talk \| contribs) ← Older edit		Latest revision as of 16:48, 18 October 2021 (view source) YihengYang (Talk \| contribs)
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	Native SsuE was a homodimeric enzyme of 58.4 kDa, which catalyzed an NAD(P)H-dependent reduction of FMN, but it was also able to reduce FAD or riboflavin. We adjusted its expression intensity in engineered bacteria, in order to enhance the bioreduction ability and enhance its tolerance to selenite, finally improve the yield of SeNPs.		Native SsuE was a homodimeric enzyme of 58.4 kDa, which catalyzed an NAD(P)H-dependent reduction of FMN, but it was also able to reduce FAD or riboflavin. We adjusted its expression intensity in engineered bacteria, in order to enhance the bioreduction ability and enhance its tolerance to selenite, finally improve the yield of SeNPs.

−	Gene ssuE comes from the ssuEADCB gene cluster, which is required for the utilization of alkanesulfonates as sulfur sources, and is expressed under conditions of sulfate or cysteine starvation. Escherichia coli BL21 also has this gene cluster. The gene sequence of this part was obtained from NCBI and its GenBank Accession number is: 8182327.	+	Gene <i>ssuE</i> comes from the <i>ssuEADCB</i> gene cluster, which is required for the utilization of alkanesulfonates as sulfur sources, and is expressed under conditions of sulfate or cysteine starvation. <i>Escherichia coli</i> BL21 also has this gene cluster. The gene sequence of this part was obtained from NCBI and its GenBank Accession number is: 8182327.