Difference between revisions of "Part:BBa K3718008"

Latest revision as of 15:55, 18 October 2021

Demonstrative circuit of tool kit part 2

An example product of our toolkit part II after cloning

After the experiments of MRP expression and rescue K.O. E. coli have proven to be successful, we will use caffeine-sensitive MRP as a testing part to model the system. Caffeine will be used to homodimerize the receptors, and is expected to induce the expression of downstream genes. This model will be used to conduct three experiments to confirm the functionality of the tool. By cloning in an antibody VHH gene against caffeine and gfp as a desired gene to be expressed, we expect the platform is activated in the presence of caffeine to express gfp, arcA and iclR, which will result in an increase in growth rate and a visible green color under UV.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 10:38, 26 September 2021 (view source) Registry (Talk \| contribs)		Latest revision as of 15:55, 18 October 2021 (view source) Stephy0521 (Talk \| contribs)
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	An example product of our toolkit part II after cloning		An example product of our toolkit part II after cloning

−	After the experiments of MRP expression and rescue K.O. E. <I>coli</I> have proven to be successful, we will use caffeine-sensitive MRP as a testing part to model the system. Caffeine will be used to homodimerize the receptors, and is expected to induce the expression of downstream genes. This model will be used to conduct three experiments to confirm the functionality of the tool. By cloning in an antibody VHH gene against caffeine and gfp as a desired gene to be expressed, we expect the platform is activated in the presence of caffeine to express gfp, arcA and iclR, which will result in an increase in growth rate and a visible green color under UV.	+	After the experiments of MRP expression and rescue K.O. E. <I>coli</I> have proven to be successful, we will use caffeine-sensitive MRP as a testing part to model the system. Caffeine will be used to homodimerize the receptors, and is expected to induce the expression of downstream genes. This model will be used to conduct three experiments to confirm the functionality of the tool. By cloning in an antibody VHH gene against caffeine and gfp as a desired gene to be expressed, we expect the platform is activated in the presence of caffeine to express gfp, <i>arcA</i> and <i>iclR</i>, which will result in an increase in growth rate and a visible green color under UV.