Difference between revisions of "Part:BBa K3868075"
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Promoter of glucose-6-phosphate isomerase gene.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pYLXP- 2-Nluc plasmid.
 
Promoter of glucose-6-phosphate isomerase gene.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pYLXP- 2-Nluc plasmid.
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===Usage===
 
  
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By codon optimization and adding a 6His-tag, the sequence suitable for expression in ''E. coli'' was constructed, and we hoped that it could reduce glyoxylic acid in ''E. coli'' to get fluorescence signal in the next processes we design.
 
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The coding sequence of target gene was inserted into an expression vectors with <partinfo>BBa_K880005</partinfo>(<partinfo>BBa_J23100</partinfo> & <partinfo>BBa_B0034</partinfo>) to obtain <partinfo>BBa_K3332056</partinfo>. We transformed the constructed plasmid into ''E. coli'' BL21 (DE3) to verify its successful heterologous expression.
 
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<html>
 
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    <figure>
 
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        <img src="https://2020.igem.org/wiki/images/2/2e/T--XMU-China--XMU-China_2020-J23100_B0034_grhpr-his-tag_B0015.png" height="150"  style="float:center">
 
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        <figcaption>
 
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        <p style="font-size:1rem">
 
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        </p>
 
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        </figcaption>
 
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    </figure>
 
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</html>
 
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:'''Fig 2.''' Gene circuit of GRHPR.
 
 
<!-- Add more about the biology of this part here-->
 
<!-- Add more about the biology of this part here-->
  

Revision as of 14:44, 18 October 2021


pYLXP-2

Promoter of glucose-6-phosphate isomerase gene.This part will go through PCR, accounting gel electrophoresis and gel recovery, and then be used as the functional module of the pYLXP- 2-Nluc plasmid.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 701
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 994
    Illegal BsaI.rc site found at 887
    Illegal SapI.rc site found at 1178