Difference between revisions of "Part:BBa K4028005"

 
Line 1: Line 1:
 +
== Profile ==
  
−
__NOTOC__
+
Name: tet promoter
−
<partinfo>BBa_K4028005 short</partinfo>
+
  
−
tet ptomoter
+
Base Pairs: 56bp
  
−
<!-- Add more about the biology of this part here
+
Origin: Escherichia coli, synthetic
−
===Usage and Biology===
+
  
−
<!-- -->
+
Properties: Inducible promoter regulated by tetracycline
−
<span class='h3bb'>Sequence and Features</span>
+
−
<partinfo>BBa_K4028005 SequenceAndFeatures</partinfo>
+
  
 +
== Usage and Biology ==
  
−
<!-- Uncomment this to enable Functional Parameter display
+
This is the naturally-occuring version of the TetR class B promoter. Note that this will promote bidirectionally. (pR1 and pR2 will promote in the forward direction; pA will promote in the reverse direction.) The three transcription start sites are not labeled, but can be found from the reference.
−
===Functional Parameters===
+
 
−
<partinfo>BBa_K4028005 parameters</partinfo>
+
We set different tetracycline concentrations to test the protein expression induced by TetR(B) promoter. In our design, the immune protein (ike4) is regulated by TetR(B) promoter. The expression of the immune protein (ike4) directly affects and is positively correlated with strain growth. Therefore, the strain growth can indicate the regulation of the TetR(B) promoter.
−
<!-- -->
+
 
 +
The results of the test are below.
 +
 
 +
[[File:T--Shanghai_Metro--parts_BBa_K4028005-tet_promoter-figure2.png|700px|thumb|center|Table 1. OD600 value of the strain D: Pus232-ike4-tke4 after 6 hours]]
 +
 
 +
[[File:T--Shanghai_Metro--parts_BBa_K4028005-tet_promoter-figure1.png|700px|thumb|center|Figure 1. Biomass Concentration curve of Pus323-tke4-ike4 Strain with tetracycline]]
 +
 
 +
The results show that concentrations at the range of 300-500µg/L are more effective. The results also proved that adding tetracycline to strain will boost the growth of strain. It determines that the optimal concentration of tetracycline is 300µg/L to 500µg/L.

Revision as of 14:40, 18 October 2021

Profile

Name: tet promoter

Base Pairs: 56bp

Origin: Escherichia coli, synthetic

Properties: Inducible promoter regulated by tetracycline

Usage and Biology

This is the naturally-occuring version of the TetR class B promoter. Note that this will promote bidirectionally. (pR1 and pR2 will promote in the forward direction; pA will promote in the reverse direction.) The three transcription start sites are not labeled, but can be found from the reference.

We set different tetracycline concentrations to test the protein expression induced by TetR(B) promoter. In our design, the immune protein (ike4) is regulated by TetR(B) promoter. The expression of the immune protein (ike4) directly affects and is positively correlated with strain growth. Therefore, the strain growth can indicate the regulation of the TetR(B) promoter.

The results of the test are below.

Table 1. OD600 value of the strain D: Pus232-ike4-tke4 after 6 hours
Figure 1. Biomass Concentration curve of Pus323-tke4-ike4 Strain with tetracycline

The results show that concentrations at the range of 300-500µg/L are more effective. The results also proved that adding tetracycline to strain will boost the growth of strain. It determines that the optimal concentration of tetracycline is 300µg/L to 500µg/L.