Difference between revisions of "Part:BBa K3838567"

Latest revision as of 14:22, 18 October 2021

6xHis Tag

It is 6x His tag. A short amino acid peptide consisting of six histidine can be used as a label for affinity chromatography for protein purification. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Data:SZU-China 2021 TEAM

During the experiment, we used His tag to purify and screen our target protein. It was successfully applied in DH5α -PJB, DH5α -PJT and Nissle 1917-PJT.

Figure 1. SDS-Page of affinity purification of transformed DH5α-pJB intracellular protein

Figure 2. SDS-Page of affinity purification of transformed DH5α-pJT intracellular protein

Figure 3. SDS-Page electrophoretic diagram of Nissle 1917-pJT intracellular protein

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3838567 short</partinfo>
-It is 6xHis tag.
+It is 6x His tag.
 A short amino acid peptide consisting of six histidine can be used as a label for affinity chromatography for protein purification.
 <!-- Add more about the biology of this part here
@@ Line 19: / Line 19: @@
 ===Data:SZU-China 2021 TEAM===
-During the experiment, we used HIs tag to purify and screen our target protein. It was successfully applied in DH5α -PJB, DH5α -PJT and Nissle 1917-PJT.
+During the experiment, we used His tag to purify and screen our target protein. It was successfully applied in DH5α -PJB, DH5α -PJT and Nissle 1917-PJT.
 [[File:T--SZU-China--BBa K3838222-JBSH3A.png|500px|center]]