Difference between revisions of "Part:BBa K3989008"

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	As we can see in figure 2, the original promoter: P<sub>esaR</sub> has only one <i>esa box</i> located at position -10 and the P<sub>esaR-C</sub> has an additional <i>esa box</i> which locates between position -10 and -35. Previous studies has shown that even with a same amount of AHL molecules and same type of the EsaR regulator, the gene expression level can still be different depending on the type of the promoter.(see figure 3)		As we can see in figure 2, the original promoter: P<sub>esaR</sub> has only one <i>esa box</i> located at position -10 and the P<sub>esaR-C</sub> has an additional <i>esa box</i> which locates between position -10 and -35. Previous studies has shown that even with a same amount of AHL molecules and same type of the EsaR regulator, the gene expression level can still be different depending on the type of the promoter.(see figure 3)
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	[[File:21 UZurich PeaR charac.jpeg|400px]][[File:21 UZurich PeaRC charac.jpeg|400px]]		[[File:21 UZurich PeaR charac.jpeg|400px]][[File:21 UZurich PeaRC charac.jpeg|400px]]

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Revision as of 14:20, 18 October 2021

Engineered quorum sensing esaR promoter: PesaR-C

A promoter that can interact with EsaR protein and inhibit downstream gene transcription.

Basic information

This part represents a promoter(P_esa) variant with two esa box, which can be bound to EsaR protein(see figure 2). esa box is a 18bps period located at a specific position of the promoter. After binding to the box, the EsaR will repress the recruitment of the RNA polymerase and inhibit the downstream transcription. However, when there are AHL molecules(such as 3OC6HSL) existing in the environment, they will interact with the EsaR and release it from the promoter, thus the transcription can be started.(Figure 1) The transcription level is controlled by the concentration of the AHL molecules.

Below is the figure of the structure of the original promoter and the engineered one.

As we can see in figure 2, the original promoter: P_esaR has only one esa box located at position -10 and the P_esaR-C has an additional esa box which locates between position -10 and -35. Previous studies has shown that even with a same amount of AHL molecules and same type of the EsaR regulator, the gene expression level can still be different depending on the type of the promoter.(see figure 3)

Sequence and Features