Difference between revisions of "Part:BBa K3799001"
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===Usage and Biology===
 
===Usage and Biology===
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This part can be used to produce csm6 protein in Ecoli host.
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This part can be used to produce csm6 protein in Ecoli host under the induction of IPTG.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 13:53, 18 October 2021


EiCsm6 under IPTG inducible promoter with double terminator

This part encodes for IPTG inducible expression of Crispr protein,csm6.It consist of coding sequence of csm6 from Enterococcus italicus(BBa-K3799000) under IPTG inducible EColi promoter Pveg(BBa_j04500) along with strong RBS(BBa_B0034) and double terminator(BBa_B0015).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 534
    Illegal XhoI site found at 587
    Illegal XhoI site found at 1274
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]