Difference between revisions of "Part:BBa K3989008"
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<partinfo>BBa_K3989008 short</partinfo>
 
<partinfo>BBa_K3989008 short</partinfo>
  
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A promoter that can interact with EsaR protein and initiate downstream gene transcription.
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A promoter that can interact with EsaR protein and inhibit downstream gene transcription.
  
 
===Basic information===
 
===Basic information===
 
<html>
 
<html>
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This part represents a promoter variant with two <i>esa box</i>(position -10 & between -10, -35), which can be bound to EsaR protein. After binding to the box, the EsaR will recruit the RNA polymerase and regulate the downstream transcription. The original promoter: P<sub>esa</sub>R has only one <i>esa box</i> located at position -10 and shows a higher transcription efficiency.
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This part represents a promoter(P<sub>esa</sub>) variant with two <i>esa box</i>(position -10 & between -10, -35), which can be bound to EsaR protein. After binding to the box, the EsaR will repress the recruitment of the RNA polymerase and inhibit the downstream transcription. However, when there are AHL molecules(such as 3OC6HSL) existing in the environment, they will interact with the EsaR and release it from the promoter, thus the transcription can be started.(Figure 1) The transcription level is controlled by the concentration of the AHL molecules.
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<figure>
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  <img src="https://static.igem.org/mediawiki/parts/b/ba/21_UZurich_EsaR_repression.jpeg">
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  <figcaption><b>Figure 1.</b> Mechanism of the EsaR being an repressor under the control of the original P<sub>esa</sub>R.[1]</figcaption>
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</figure>
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<figure>
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  <img src="https://static.igem.org/mediawiki/parts/a/a1/21_UZurich_PesaRC.jpeg">
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  <img src="https://static.igem.org/mediawiki/parts/f/fb/21_UZurich_PesaR.jpeg">
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  <figcaption><b>Figure 1.</b> The structure of P<sub>esaR-C</sub>RC.[2]</figcaption>
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</figure>
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The original promoter: P<sub>esa</sub>R has only one <i>esa box</i> located at position -10 and shows a higher transcription efficiency.  
  
  

Revision as of 13:44, 18 October 2021


Engineered quorum sensing esaR promoter: PesaR-C

A promoter that can interact with EsaR protein and inhibit downstream gene transcription.

Basic information

This part represents a promoter(Pesa) variant with two esa box(position -10 & between -10, -35), which can be bound to EsaR protein. After binding to the box, the EsaR will repress the recruitment of the RNA polymerase and inhibit the downstream transcription. However, when there are AHL molecules(such as 3OC6HSL) existing in the environment, they will interact with the EsaR and release it from the promoter, thus the transcription can be started.(Figure 1) The transcription level is controlled by the concentration of the AHL molecules.

Figure 1. Mechanism of the EsaR being an repressor under the control of the original PesaR.[1]
Figure 1. The structure of PesaR-CRC.[2]
The original promoter: PesaR has only one esa box located at position -10 and shows a higher transcription efficiency. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 281
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]