Difference between revisions of "Part:BBa K3852007"
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<partinfo>BBa_K3852007 short</partinfo> | <partinfo>BBa_K3852007 short</partinfo> | ||
| − | + | The promoter Pfus(activated by Ste12 in the MAPK pathway) was linked to the reporter gene green fluorescent protein gene eGFP and CYC1T to form the detection gene circuit, which was carried into S. cerevisiae cells by shuttle vector pRS42K. Positive colonies were screened for ambenzyl antibody and verified by PCR. Finally, the plasmid was extracted and sequenced to confirm whether the correct plasmid was imported into the colony. | |
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| + | [[File:T--BIT-China--Engeering 16.png|550px|thumb|center|Figure1 ]] | ||
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Latest revision as of 13:38, 18 October 2021
Pfus+eGFP+CYC1t
The promoter Pfus(activated by Ste12 in the MAPK pathway) was linked to the reporter gene green fluorescent protein gene eGFP and CYC1T to form the detection gene circuit, which was carried into S. cerevisiae cells by shuttle vector pRS42K. Positive colonies were screened for ambenzyl antibody and verified by PCR. Finally, the plasmid was extracted and sequenced to confirm whether the correct plasmid was imported into the colony.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]