Difference between revisions of "Part:BBa K3805209:Design"
(→Source) |
|||
| Line 17: | Line 17: | ||
3.lacI- LVA：https://parts.igem.org/Part:BBa_C0012 | 3.lacI- LVA：https://parts.igem.org/Part:BBa_C0012 | ||
4.mcherry-ompt:https://parts.igem.org/Part:BBa_K3805886 | 4.mcherry-ompt:https://parts.igem.org/Part:BBa_K3805886 | ||
| − | |||
| − | |||
| − | |||
===References=== | ===References=== | ||
Revision as of 13:03, 18 October 2021
Bi-stable Switch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1287
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This is a bistable switch designed for the "guard" bacteria in our project. In the presence of AIP, the expression of mcherry-ompt will be activated. And it will be secreted outside the cell, stimulate the expression of ccdB and kill the "cheater" bacteria.In the absence of AIP, the bistable switch enables the "guard" bacteria to work like ordinary bacteria to produce the target protein.
Source
1.trc promoter：ptr99a plasmid 2.P2 promoter：https://parts.igem.org/Part:BBa_I746104 3.lacI- LVA：https://parts.igem.org/Part:BBa_C0012 4.mcherry-ompt:https://parts.igem.org/Part:BBa_K3805886