Difference between revisions of "Part:BBa K4088888"

Revision as of 12:51, 18 October 2021

dCas13a-Clact

С-terminal fragment of β-lactamase fused with dCas13.
β-Lactamase (AmpR) -
C-terminal fragment of β-lactamase -

dCAS13 is a catalytically dead Cas13a enzyme(formerly C2c2). The Cas13 family of enzymes includes at least 4 families, for example Cas13a, which is used in this biobrick. dCas13a interacts with gRNA about 65 nucleotides in length. dCas13a protein complexes with gRNA detect complementary RNA in the sample. Cas13 based detection is specific and can be customized to recognize single nucleotides on the target RNA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1580
Illegal BglII site found at 1790
Illegal BglII site found at 2057
Illegal BglII site found at 2822
Illegal XhoI site found at 3751
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 β-Lactamase (AmpR) - <br/>
 C-terminal fragment of β-lactamase - <br/><br/>
-dCAS13 is a catalytically dead Cas13 enzyme. The diverse Cas13 family contains at least four known subtypes, including Cas13a (formerly C2c2), which we used. Cas13 uses ~ 64 nucleotide guide RNA to encode target specificity. Cas13 protein complexes with gRNA detect complementary RNA in the sample. Cas13 based detection is specific and can be customized to recognize single nucleotides on the target RNA.<br/><br/>
+dCAS13 is a catalytically dead Cas13a enzyme(formerly C2c2). The Cas13 family of enzymes includes at least 4 families, for example Cas13a, which is used in this biobrick. dCas13a interacts with gRNA about 65 nucleotides in length. dCas13a protein complexes with gRNA detect complementary RNA in the sample. Cas13 based detection is specific and can be customized to recognize single nucleotides on the target RNA.<br/><br/>