Difference between revisions of "Part:BBa K3776020"

Revision as of 12:36, 18 October 2021

panS-SPIN-davBA-AK

This part allows This plasmid contains the INTEGRATE system () cloned into the panS-loxP-MCS shuttle vector (). A feedback insensitive aspartate kinase () for overproduction of lysine, DavB () and DavA () for synthesis of 5-aminovalerate (5AVA) have been cloned as the donor DNA in the INTEGRATE system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7311
Illegal NheI site found at 9398
Illegal NheI site found at 13516
Illegal NotI site found at 33
Illegal NotI site found at 15236
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 206
Illegal BglII site found at 6737
Illegal BglII site found at 7528
Illegal BglII site found at 8058
Illegal BglII site found at 13303
Illegal BglII site found at 16325
Illegal BglII site found at 19280
Illegal BamHI site found at 6
Illegal BamHI site found at 5880
Illegal BamHI site found at 14431
Illegal XhoI site found at 5663
Illegal XhoI site found at 14414
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2378
Illegal NgoMIV site found at 16485
Illegal NgoMIV site found at 18252
Illegal AgeI site found at 4103
Illegal AgeI site found at 13435
Illegal AgeI site found at 15892
Illegal AgeI site found at 16141
Illegal AgeI site found at 17476
Illegal AgeI site found at 19479
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 5846
Illegal BsaI.rc site found at 5820
Illegal SapI.rc site found at 8814

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+This part allows  This plasmid contains the INTEGRATE system () cloned into the panS-loxP-MCS shuttle vector (). A feedback insensitive aspartate kinase () for overproduction of lysine, DavB () and DavA () for synthesis of 5-aminovalerate (5AVA) have been cloned as the donor DNA in the INTEGRATE system.
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