Difference between revisions of "Part:BBa K3893030"

Revision as of 12:16, 18 October 2021

Population control device (QS-based lysis protein oscilator)

Figure 1. SBOL Visual representation of the device

This device is an AHL induced transcriptional unit to express phiX174 lysis protein together with a TU for the constitutive expression of LuxI. It was assembled with a one-pot Level 2 Golden Gate reaction using BsmBI type IIS endonuclease.

This device is composed of the following standardized composite parts:

BBa_K3893028: a Level 2 transcriptional unit constitutively expressing luxI gene (Golden Braid compatible)
BBa_K3893029: a Level 2 transcriptional unit expresing phiX174 lysis protein under the control of the pux promoter (Golden Braid compatible)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 956
Illegal NheI site found at 979
Illegal NheI site found at 1914
Illegal NheI site found at 1937
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3893030 short</partinfo>
-[[File:T--Ecuador--K3893030_sbolv.png|500px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the device]]
+[[File:T--Ecuador--K3893030_sbolv.png|700px|thumb|right|alt=domestication.|Figure 1. SBOL Visual representation of the device]]
 This device is an AHL induced transcriptional unit to express phiX174 lysis protein together with a TU for the constitutive expression of LuxI. It was assembled with a one-pot Level 2 Golden Gate reaction using BsmBI type IIS endonuclease.