Difference between revisions of "Part:BBa K3740009"
(→‎2021 SZPT-China)
Line 15: Line 15:
 
=2021 SZPT-China=
 
=2021 SZPT-China=
 
<h3>Biology</h3>
 
<h3>Biology</h3>
−
<p>The S gene of lambda bacteriophage has the characteristics of a double initiation motif,which can encode two proteins with opposite functions,S107 and S105. Among them,S105 plays the function of perforin,S107 also participates in the formation of transmembrane pores when triggering lysis,but before triggering lysis,it has the function of antagonizing S105 to prevent the premature formation of transmembrane pores,so S107 is called anti-perforin. The interaction between S105 and S107 is inseparable,collectively referred to as S protein. The structure directed initiation (sdi)of the S gene to form or open the stem-loop structure at the ribosome binding site (RBS)sequence GGGGG encoding S107,thereby regulating the synthesis ratio of the two proteins S107 and S105.</p>
+
<p>The S gene of lambda bacteriophage has the characteristics of a double initiation motif, which can encode two proteins with opposite functions, S107 and S105. Among them, S105 plays the function of holin. It accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space. </p>
 
<h3>References</h3>
 
<h3>References</h3>
−
[1] Yibo S, Jianhe S, Shi Y, et al. Current advance in the topological structure and function of holin encoded by bacteriophage lambda[J]. Acta Microbiologica Sinica, 2012, 52(2):141-145.
+
[1] Yibo S, Jianhe S, Shi Y, et al. Current advance in the topological structure and function of holin encoded by bacteriophage lambda[J]. Acta Microbiologica Sinica, 2012, 52(2):141-145

Revision as of 11:58, 18 October 2021


Holin S

Description

Lysis of the engineered bacteria

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


2021 SZPT-China

Biology

The S gene of lambda bacteriophage has the characteristics of a double initiation motif, which can encode two proteins with opposite functions, S107 and S105. Among them, S105 plays the function of holin. It accumulates harmlessly in the cytoplasmic membrane until it reaches a critical concentration that triggers the formation of micron-scale pores (holes) causing host cell membrane disruption and endolysin escape into the periplasmic space.

References

[1] Yibo S, Jianhe S, Shi Y, et al. Current advance in the topological structure and function of holin encoded by bacteriophage lambda[J]. Acta Microbiologica Sinica, 2012, 52(2):141-145