Difference between revisions of "Part:BBa K3771050"
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| − | <br>We compared the relative fluorescence intensity of sfGFP expressed by different promoters under the stimulation by different concentrations of paraquat. As expected, the sfGFP expression level of J23100 shows no difference between the groups with and without paraquat. <i>P<sub>soxS</sub></i> with its activator, SoxR (BBa_K37710XX), is highly activated under oxidative stress and has the highest gene expression level compared to J23100, <i>P<sub>katG</sub></i> (BBa_K37710XX), and <i>P<sub>soxS</sub></i> (BBa_K37710XX) solely ( | + | <br>We compared the relative fluorescence intensity of sfGFP expressed by different promoters under the stimulation by different concentrations of paraquat. As expected, the sfGFP expression level of J23100 shows no difference between the groups with and without paraquat. <i>P<sub>soxS</sub></i> with its activator, SoxR (BBa_K37710XX), is highly activated under oxidative stress and has the highest gene expression level compared to J23100, <i>P<sub>katG</sub></i> (BBa_K37710XX), and <i>P<sub>soxS</sub></i> (BBa_K37710XX) solely (Fig. 1). |
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| − | <img src="https://static.igem.org/mediawiki/parts/c/c2/T--NCKU_Tainan--sfGFP_Expression_%28PQ_%404.5h%29.png" style="width: | + | <img src="https://static.igem.org/mediawiki/parts/c/c2/T--NCKU_Tainan--sfGFP_Expression_%28PQ_%404.5h%29.png" style="width:45%;"> |
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| − | <p align="center"> Fig.1. Comparison of different promoter strengths by relative fluorescence intensity after 4.5-hour incubation with paraquat in various concentrations. | + | <p align="center"> Fig. 1. Comparison of different promoter strengths by relative fluorescence intensity after 4.5-hour incubation with paraquat in various concentrations. |
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Revision as of 11:41, 18 October 2021
J23100-sfGFP
Description
This composite part consists of the constitutive promoter J23100 (BBa_J23100) and sfGFP (BBa_K1321337). It serves as a positive control for another two oxidative-stress-induced promoters in our design, which should not alter its downstream gene expression under oxidative stress.
Usage and Biology
We compared the relative fluorescence intensity of sfGFP expressed by different promoters under the stimulation by different concentrations of paraquat. As expected, the sfGFP expression level of J23100 shows no difference between the groups with and without paraquat. PsoxS with its activator, SoxR (BBa_K37710XX), is highly activated under oxidative stress and has the highest gene expression level compared to J23100, PkatG (BBa_K37710XX), and PsoxS (BBa_K37710XX) solely (Fig. 1).
Fig. 1. Comparison of different promoter strengths by relative fluorescence intensity after 4.5-hour incubation with paraquat in various concentrations.