Difference between revisions of "Part:BBa K3805886"

Revision as of 09:13, 18 October 2021

OmpT-mCherry

OmpT protease is highly expressed in E. coli, and the signal peptide of the amino-terminal 1 9 amino acid residues of its protein precursor is recognized, sheared and processed by the signal skin enzyme during transmembrane and then secreted into the peripheral lumen of the cell, so the signal skin sequence can be used to construct a secretory prokaryotic expression vector. By adding the ompT signal peptide sequence before the Mcherry gene expressed in the vector, the amino-terminal end of Mcherry can be secreted outside the cell under the guidance of the OmpT signal peptide.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3805886 short</partinfo>
-The ompt protease is highly expressed in E. coli and has a secretory effect, and can be processed into an outer membrane protease that is secreted extracellularly. The addition of the ompt gene before the Mcherry gene, expressed in a vector, allows the originally endocrine Mcherry, guided by Ompt, to be secreted extracellularly.
+OmpT protease is highly expressed in E. coli, and the signal peptide of the amino-terminal 1 9 amino acid residues of its protein precursor is recognized, sheared and processed by the signal skin enzyme during transmembrane and then secreted into the peripheral lumen of the cell, so the signal skin sequence can be used to construct a secretory prokaryotic expression vector. By adding the ompT signal peptide sequence before the Mcherry gene expressed in the vector, the amino-terminal end of Mcherry can be secreted outside the cell under the guidance of the OmpT signal peptide.
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