Difference between revisions of "Part:BBa K3875030"
| Line 6: | Line 6: | ||
Phyb-mazE-T1<br> | Phyb-mazE-T1<br> | ||
| − | + | Biology:<br> | |
Phyb :the promoter of the hyb operon<br> | Phyb :the promoter of the hyb operon<br> | ||
mazE :antitoxic gene<br> | mazE :antitoxic gene<br> | ||
| − | + | Design:<br> | |
We connect MazE with the <i>phyb</i> promoter to ensure that the <i>mazE</i> protein is expressed only under anaerobic conditions, and once the engineered bacteria are exposed to oxygen, drastic changes in oxygen concentration will cause the <i>phyb</i> promoter expression to fail, thus preventing the expression of antitoxins, which in turn will lead to the death of the engineering bacteria<br> | We connect MazE with the <i>phyb</i> promoter to ensure that the <i>mazE</i> protein is expressed only under anaerobic conditions, and once the engineered bacteria are exposed to oxygen, drastic changes in oxygen concentration will cause the <i>phyb</i> promoter expression to fail, thus preventing the expression of antitoxins, which in turn will lead to the death of the engineering bacteria<br> | ||
| − | + | Reference:<br> | |
Richard, D. J., Sawers, G., Sargent, F., McWalter, L., & Boxer, D. H. (1999). Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Microbiology (Reading, England), 145 ( Pt 10), 2903–2912. | Richard, D. J., Sawers, G., Sargent, F., McWalter, L., & Boxer, D. H. (1999). Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Microbiology (Reading, England), 145 ( Pt 10), 2903–2912. | ||
Revision as of 08:18, 18 October 2021
Usage and Biology
Phyb-mazE-T1
Biology:
Phyb :the promoter of the hyb operon
mazE :antitoxic gene
Design:
We connect MazE with the phyb promoter to ensure that the mazE protein is expressed only under anaerobic conditions, and once the engineered bacteria are exposed to oxygen, drastic changes in oxygen concentration will cause the phyb promoter expression to fail, thus preventing the expression of antitoxins, which in turn will lead to the death of the engineering bacteria
Reference:
Richard, D. J., Sawers, G., Sargent, F., McWalter, L., & Boxer, D. H. (1999). Transcriptional regulation in response to oxygen and nitrate of the operons encoding the [NiFe] hydrogenases 1 and 2 of Escherichia coli. Microbiology (Reading, England), 145 ( Pt 10), 2903–2912.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 241
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]