Difference between revisions of "Part:BBa K3875023"

 
 
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<partinfo>BBa_K3875023 short</partinfo>
 
  
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===Usage and Biology===
 
===Usage and Biology===
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23: sgRNA-R
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Using a strand of single guide RNA (sgRNA), the Cas9 protein is able to search a cellular genome and induce double stranded breaks at a target sequence complementary to the sgRNA that can then be modified.
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<img src="https://static.igem.org/mediawiki/parts/6/6a/T--BUCT--Plasmid_profile_of_Target_fadR_N20.png"width="640px";height="30px"/><br>
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Latest revision as of 08:14, 18 October 2021


Usage and Biology

23: sgRNA-R Using a strand of single guide RNA (sgRNA), the Cas9 protein is able to search a cellular genome and induce double stranded breaks at a target sequence complementary to the sgRNA that can then be modified.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1
  • 1000
    COMPATIBLE WITH RFC[1000]