Difference between revisions of "Part:BBa K3784006:Design"

 
(→‎Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
mull
 
−
 
  
 +
Use gibson recombination to ligate it to the plasmid.
  
 
===Source===
 
===Source===

Latest revision as of 08:08, 18 October 2021


Fld


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3649
    Illegal EcoRI site found at 3721
    Illegal XbaI site found at 4570
    Illegal SpeI site found at 5354
    Illegal SpeI site found at 5682
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3649
    Illegal EcoRI site found at 3721
    Illegal NheI site found at 1269
    Illegal SpeI site found at 5354
    Illegal SpeI site found at 5682
    Illegal NotI site found at 2374
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3649
    Illegal EcoRI site found at 3721
    Illegal BglII site found at 2528
    Illegal XhoI site found at 111
    Illegal XhoI site found at 1222
    Illegal XhoI site found at 6204
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3649
    Illegal EcoRI site found at 3721
    Illegal XbaI site found at 4570
    Illegal SpeI site found at 5354
    Illegal SpeI site found at 5682
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3649
    Illegal EcoRI site found at 3721
    Illegal XbaI site found at 4570
    Illegal SpeI site found at 5354
    Illegal SpeI site found at 5682
    Illegal AgeI site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use gibson recombination to ligate it to the plasmid.

Source

Clostridium sporogenes

References