Difference between revisions of "Part:BBa K3784001:Design"
(→Source) |
(→Design Notes) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | Use gibson recombination to ligate it to the plasmid. | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Latest revision as of 07:59, 18 October 2021
fldB (Encoding 2-hydroxyacyl-CoA dehydratase protein family)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1169
Illegal EcoRI site found at 1241 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1169
Illegal EcoRI site found at 1241 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1169
Illegal EcoRI site found at 1241
Illegal BglII site found at 48 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1169
Illegal EcoRI site found at 1241 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1169
Illegal EcoRI site found at 1241 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Use gibson recombination to ligate it to the plasmid.
Source
From fld gene cluster of Clostridium sporogenes