Difference between revisions of "Part:BBa K3784001:Design"

(Source)
(Design Notes)
 
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===Design Notes===
 
===Design Notes===
null
+
Use gibson recombination to ligate it to the plasmid.
 
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+
  
 
===Source===
 
===Source===

Latest revision as of 07:59, 18 October 2021


fldB (Encoding 2-hydroxyacyl-CoA dehydratase protein family)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
    Illegal BglII site found at 48
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1169
    Illegal EcoRI site found at 1241
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use gibson recombination to ligate it to the plasmid.

Source

From fld gene cluster of Clostridium sporogenes

References