Difference between revisions of "Part:BBa K4083008"
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<partinfo>BBa_K4083008 short</partinfo> | <partinfo>BBa_K4083008 short</partinfo> | ||
| − | + | This plasmid consists of four parts: RPGDuo2 plasmid; nadE, rhlA, and rhlB coding sequences (https://parts.igem.org/Part:BBa_K4083000, https://parts.igem.org/Part:BBa_K4083004, https://parts.igem.org/Part:BBa_K4083006, https://parts.igem.org/Part:BBa_K4083007). | |
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| + | By inserting this plasmid, we predicted the effective rhamnolipid production by Pseudomonas putida. <!-- Add more about the biology of this part here | ||
| + | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 07:54, 18 October 2021
RPGDuo2 plasmid with nadE and rhlBA genes
This plasmid consists of four parts: RPGDuo2 plasmid; nadE, rhlA, and rhlB coding sequences (https://parts.igem.org/Part:BBa_K4083000, https://parts.igem.org/Part:BBa_K4083004, https://parts.igem.org/Part:BBa_K4083006, https://parts.igem.org/Part:BBa_K4083007).
By inserting this plasmid, we predicted the effective rhamnolipid production by Pseudomonas putida.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 5565
Illegal BamHI site found at 6125
Illegal XhoI site found at 1757
Illegal XhoI site found at 2024
Illegal XhoI site found at 6301 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1949
Illegal NgoMIV site found at 2019
Illegal NgoMIV site found at 2240
Illegal NgoMIV site found at 4400 - 1000COMPATIBLE WITH RFC[1000]