Difference between revisions of "Part:BBa K3784000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Use gibson recombination to ligate it to the plasmid | + | Use gibson recombination to ligate it to the plasmid. |
===Source=== | ===Source=== | ||
Latest revision as of 07:43, 18 October 2021
acdA (encoding 3-(aryl) acrylate reductase)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 291
Illegal SpeI site found at 619 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 291
Illegal SpeI site found at 619 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1141
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 291
Illegal SpeI site found at 619 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 291
Illegal SpeI site found at 619 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Use gibson recombination to ligate it to the plasmid.
Source
Clostridium sporogenes