Difference between revisions of "Part:BBa K3784000:Design"

 
(Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
non
+
Use gibson recombination to ligate it to the plasmid
 
+
 
+
  
 
===Source===
 
===Source===

Revision as of 07:43, 18 October 2021


acdA (encoding 3-(aryl) acrylate reductase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 291
    Illegal SpeI site found at 619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 291
    Illegal SpeI site found at 619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1141
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 291
    Illegal SpeI site found at 619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 291
    Illegal SpeI site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Use gibson recombination to ligate it to the plasmid

Source

Clostridium sporogenes

References