Difference between revisions of "Part:BBa K792002:Experience"

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'''Successfully''' used in <partinfo>BBa_K792010</partinfo>, to secrete a Trp-riched peptide. Check <partinfo>BBa_K792010</partinfo> entry to get more information about the experience.
 
'''Successfully''' used in <partinfo>BBa_K792010</partinfo>, to secrete a Trp-riched peptide. Check <partinfo>BBa_K792010</partinfo> entry to get more information about the experience.
 
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{|width='80%' style='border:1px solid gray'
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<partinfo>BBa_K792002 AddReview 5</partinfo>
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<I>CWChew</I>
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|width='60%' valign='top'|
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Successfully used in <partinfo>BBa_K3927018</partinfo>, to secrete Human Beta Defensin 2. Characterization of part performance was also done using a GFP reporter.
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https://2021.igem.org/wiki/images/thumb/6/6e/T--NUS_Singapore--results_tab1_2.png/800px-T--NUS_Singapore--results_tab1_2.png
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<i>Figure 1: Image of the media supernatant, resuspended cells and blank control media under gel visualizer blue light, media and supernatant are clearly fluorescent compared to the control media.</i>
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https://2021.igem.org/wiki/images/thumb/5/55/T--NUS_Singapore--results_tab1_1.png/800px-T--NUS_Singapore--results_tab1_1.png
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<i>Figure 2: mFa-GFP construct assembled to test the performance of this secretion tag.</i>
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https://2021.igem.org/wiki/images/thumb/c/cf/T--NUS_Singapore--results_tab1_3.png/800px-T--NUS_Singapore--results_tab1_3.png
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<i>Figure 3: GFP absorbance of the supernatant and cells of both induced and uninduced pGmFaGFP-H (BY4741), after normalization to blank media. Induced cells secreted approximately 42% of the GFP, while uninduced cells produced and secreted significantly less GFP than induced cells.</i>
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<!-- Template for a user review
 
<!-- Template for a user review

Revision as of 04:43, 18 October 2021

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K792002

User Reviews

UNIQ2ec167f6031b6e93-partinfo-00000000-QINU

•••••

manugimenez

Successfully used in BBa_K792010, to secrete a Trp-riched peptide. Check BBa_K792010 entry to get more information about the experience.

•••••

CWChew

Successfully used in BBa_K3927018, to secrete Human Beta Defensin 2. Characterization of part performance was also done using a GFP reporter.

800px-T--NUS_Singapore--results_tab1_2.png

Figure 1: Image of the media supernatant, resuspended cells and blank control media under gel visualizer blue light, media and supernatant are clearly fluorescent compared to the control media.

800px-T--NUS_Singapore--results_tab1_1.png

Figure 2: mFa-GFP construct assembled to test the performance of this secretion tag.

800px-T--NUS_Singapore--results_tab1_3.png

Figure 3: GFP absorbance of the supernatant and cells of both induced and uninduced pGmFaGFP-H (BY4741), after normalization to blank media. Induced cells secreted approximately 42% of the GFP, while uninduced cells produced and secreted significantly less GFP than induced cells.


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UNIQ2ec167f6031b6e93-partinfo-00000006-QINU