Difference between revisions of "Part:BBa K3875019"

 
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<partinfo>BBa_K3875019 short</partinfo>
 
  
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more information will be provided later.
 
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===Usage and Biology===
 
===Usage and Biology===
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It is a bacterial promoter (Registry of Standard Biological Parts BBa_J23119) that modified to end with an SpeI site. J23119 is the "consensus" promoter sequence and the strongest member of the family. The sgRNA will transcribe from the J23119(SpeI) promoter.<br>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 03:54, 18 October 2021


Usage and Biology

It is a bacterial promoter (Registry of Standard Biological Parts BBa_J23119) that modified to end with an SpeI site. J23119 is the "consensus" promoter sequence and the strongest member of the family. The sgRNA will transcribe from the J23119(SpeI) promoter.
Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 30
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal SpeI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 30
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 30
  • 1000
    COMPATIBLE WITH RFC[1000]