Difference between revisions of "Part:BBa K3888004"

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<partinfo>BBa_K3888004 short</partinfo>
 
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HoxK1 coding gene with E. coli signal sequence
 
HoxK1 coding gene with E. coli signal sequence
 
This basic part is used to achieve heterologous expression of small subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] HoxK1 encodes the small subunit of hydrogenase mentioned above, and together with HoxG1(BBa_K3888003), to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011).
 
This basic part is used to achieve heterologous expression of small subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] HoxK1 encodes the small subunit of hydrogenase mentioned above, and together with HoxG1(BBa_K3888003), to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011).

Revision as of 03:44, 18 October 2021


HoxK1


HoxK1 coding gene with E. coli signal sequence This basic part is used to achieve heterologous expression of small subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] HoxK1 encodes the small subunit of hydrogenase mentioned above, and together with HoxG1(BBa_K3888003), to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011). T--SJTang--BBa K3888011-1.png

Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 940
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]