Difference between revisions of "Part:BBa K3888004"
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<partinfo>BBa_K3888004 short</partinfo> | <partinfo>BBa_K3888004 short</partinfo> | ||
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HoxK1 coding gene with E. coli signal sequence | HoxK1 coding gene with E. coli signal sequence | ||
| + | This basic part is used to achieve heterologous expression of small subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] HoxK1 encodes the small subunit of hydrogenase mentioned above, and together with HoxG1(BBa_K3888003), to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011). | ||
| + | [[File:T--SJTang--BBa_K3888011-1.png]] | ||
| + | ==Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.== | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 03:44, 18 October 2021
HoxK1
HoxK1 coding gene with E. coli signal sequence
This basic part is used to achieve heterologous expression of small subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] HoxK1 encodes the small subunit of hydrogenase mentioned above, and together with HoxG1(BBa_K3888003), to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011).
Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 940
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]