Difference between revisions of "Part:BBa K3927006:Design"

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===Description===
 
===Description===
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LacI is a bacterial repressor commonly used in genetic circuits [1]. It has also been shown to be able to repress it’s cognate LacO sequence when expressed in S.cerevisiae [2]. The NUS iGEM 2021 team thus added a yeast optimized LacI to be able to control synthetic promoters in a repressible manner.
 
  
 
===Source===
 
===Source===

Latest revision as of 03:21, 18 October 2021


yeLacI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 253
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1044
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 253
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 253
    Illegal NgoMIV site found at 595
  • 1000
    COMPATIBLE WITH RFC[1000]


Description

Source

NIL

References