Difference between revisions of "Part:BBa K3888003"
Line 3: | Line 3: | ||
<partinfo>BBa_K3888003 short</partinfo> | <partinfo>BBa_K3888003 short</partinfo> | ||
− | HoxG1 coding gene | + | HoxG1 coding gene<br> |
− | + | This basic part is used to achieve heterologous expression of large subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] HoxG1 encodes the large subunit of hydrogenase mentioned above, and together with HoxK1(BBa_K3888004), to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011). | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 02:50, 18 October 2021
HoxG1
HoxG1 coding gene
This basic part is used to achieve heterologous expression of large subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] HoxG1 encodes the large subunit of hydrogenase mentioned above, and together with HoxK1(BBa_K3888004), to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 450
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 597
Illegal BamHI site found at 1512
Illegal XhoI site found at 1834 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450
Illegal AgeI site found at 1728 - 1000COMPATIBLE WITH RFC[1000]