Difference between revisions of "Part:BBa K3927001"
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===Design=== | ===Design=== | ||
− | + | Blue light induced activation of this promoter is dependent on simultaneous expression of an EL222 based transcription factor containing a nuclear localization sequence, and an activation domain. | |
===Characterization=== | ===Characterization=== |
Revision as of 02:41, 18 October 2021
C120-CYC
This part encodes for a truncated CYCp core promoter with C120 sequence replacing the native upstream activating sequence.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 104
Illegal SpeI site found at 79 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 86
Illegal SpeI site found at 79 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 104
Illegal SpeI site found at 79 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 104
Illegal SpeI site found at 79 - 1000COMPATIBLE WITH RFC[1000]
Description
Usage
Design
Blue light induced activation of this promoter is dependent on simultaneous expression of an EL222 based transcription factor containing a nuclear localization sequence, and an activation domain.
Characterization
References
1. Motta-Mena, L. B., Reade, A., Mallory, M. J., Glantz, S., Weiner, O. D., Lynch, K. W., & Gardner, K. H. (2014). An optogenetic gene expression system with rapid activation and deactivation kinetics. Nature chemical biology, 10(3), 196–202. https://doi.org/10.1038/nchembio.1430
2. Benzinger D, Khammash M. Pulsatile inputs achieve tunable attenuation of gene expression variability and graded multi-gene regulation. Nat Commun. 2018 Aug 30;9(1):3521. doi: 10.1038/s41467-018-05882-2. PMID: 30166548; PMCID: PMC6117348